Inhibition by Thyroxine of Enzymes requiring Pyridoxal-5-Phosphate
نویسندگان
چکیده
منابع مشابه
Use of inhibitors to study reactions catalyzed by enzymes requiring pyridoxal phosphate as coenzyme*
The stereochemistry of a variety of pyridoxal phosphate-mediated enzymic reactions has been studied using enzyme inhibitors that are stereospecifically labeled in the b-position with deuterium. A versatile synthesis has been developed to prepare a wide variety of stereospecifically labeled Dand L-amino acids and inhibitors. Investigation of the “turnover” of b-chloro-D-alanine and Dand L-serine...
متن کاملPLPMDB: Pyridoxal-5'-phosphate dependent enzymes mutants database
SUMMARY The searchable mutant database PLPMDB has been developed to provide rapid and simple access to relevant mutant information on pyridoxal-5'-phosphate dependent enzymes. All data have been extracted from publications and publicly available databases, then organized in a relational database to enable searching via a web-based search form. The current version of PLPMDB contains 688 mutants ...
متن کاملBinding of Pyridoxal 5'-Phosphate
1. The a and ,B subforms of aspartate aminotransferase were purified from pig heart. 2. The a subform contained 2mol of pyridoxal 5'-phosphate. The apo-(a subform) could be fully reactived by combination with 2mol of cofactor. 3. The protein fluorescence of the apo(a subform) decreased non-linearly with increase in enzyme activity and concentration of bound cofactor. 4. It is concluded that the...
متن کاملTransfer of pyridoxal 5'-phosphate from albumin-pyridoxal 5'-phosphate complex to apo-aspartate aminotransferase.
Pyridoxal 5•L-phosphate (PLP) is known to combine with bovine serum albumin to form a(1:1) complex which scarcely dissociates, even when subjected to intensive dialysis. When this complex was incubated with apo-aspartate aminotransferase (apoGOT) for an appropriate time and the preincubated mixture then submitted to the usual GOT assay, the appearance of GOT activity was obviously confirmed, in...
متن کاملA possible mechanism for the inhibition of blood platelet aggregation by pyridoxal-5'-phosphate.
Pyridoxal-5'-phosphate (PLP) the coenzyme form of vitamin Bg. has been shown to inhibit platelet aggegation [l-31 but the mechanism of its inhibitory action remains to be elucidated. Because of its negative charge and hydrophilic nature PLP does not cross the cell membrane and has been used as a probe for several membrane systems [3]. Since PLP inhibits platelet activation induced by a variety ...
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ژورنال
عنوان ژورنال: Nature
سال: 1957
ISSN: 0028-0836,1476-4687
DOI: 10.1038/179968a0